Rockefeller University Research Assistant in New York, New York
Job Title Research Assistant
Employment Status Full Time
Laboratory / Department Human Genetics of Infectious Diseases
The Laboratory of Human Genetics of Infectious Diseases studies the genetics of human predisposition to pediatric infectious diseases, particularly mycobacterial diseases, invasive pneumococcal diseases and herpes simplex encephalitis. It aims to identify Mendelian 'holes' in the immune defense of otherwise healthy children who are susceptible to specific infectious diseases, work that has profound implications for and has resulted in a paradigm shift in human clinical medicine and fundamental immunology. In the past decade, the laboratory has uncovered that single genetic lesions in children confer severe and selective vulnerability to certain illnesses, whereas corresponding infections in adults result more from polygenetic inheritance. This work not only blurs the distinction between patient-based Mendelian genetics and population-based complex genetics but has also provided experimental support for a unified theory of human infectious diseases.
Working closely under the supervision of senior scientists, the Research Assistant will engage in genetic dissection of human infectious diseases. Will perform basic and advanced laboratory experiments in molecular biology and immunology to characterize various Toll-like receptor 3 (TLR3) mutations and to study the human immune response to virus infection. Will be responsible for measuring innate immune responses using molecular biology techniques such as DNA cloning, qPCR, Western blotting, ELISA, and microscopy. Will use CRISPR/Cas9 for gene editing, generate human cell lines expressing various mutants or wild-type TLR3 and assess their responsiveness to TLR3 against stimulation, and quantify virus replication in patient-specific human cell lines.
Bachelor's degree in science required; educational emphasis in biochemistry, molecular genetics, immunology, or related field preferred. Experience with tissue culture preffered. Must be detail-oriented, have a strong sense of responsibility and be able to work as part of a team in a fast-paced environment. Strong interpersonal and communication skills, computer proficiency and excellent record keeping required.