Rockefeller University Research Assistant in New York, New York
Job Title Research Assistant
Laboratory / Department Laboratory of Molecular Biology
Research in the laboratory of molecular biology aims to identify the genes, circuits, cells, macromolecular assemblies and individual molecules that contribute to the function and dysfunction of the mammalian brain. Scientists in the laboratory of molecular biology have developed a suite of novel approaches based on the manipulation of bacterial artificial chromosomes (BACs) to investigate the histological and functional complexities of the mammalian brain in vivo and to understand how these mechanisms become dysfunctional in disease.
The Research Assistant will work on a project studying the molecular basis of selective cellular vulnerability in Huntington's disease. The ultimate goal of this project is to identify novel strategies to delay onset and/or slow progression of Huntingdon's disease. The Research Assistant will be responsible for the preparation of human brain samples for next generation sequencing. Responsibilities include subcellular fractionation, isolation of cell components with antibodies or DNA probes (via FACs), RNA extraction, cDNA preparation, PCR, gel electrophoresis and library preparation. Additional responsibilities will include preparing genetic material for BeadChip methylation assays and trinucleotide repeat sizing. Will work with contract testing laboratories to ensure proper amounts of sample and control materials are sent.
Bachelor's degree in science required; educational emphasis in neuroscience or biology preferred. Previous experience in situ hybridization and knowledge of brain anatomy is a plus. Must have computer skills and familiarity with NCBI database tools. Must be organized, have good communication skills and keep clear accurate records. Familiarity with established profiling assays such as ATAC-Seq, bisulfite-Seq and PX-bisulfite Seq to elucidate the epigenetic properties of the DNA preferred.
The Rockefeller University is an Equal Opportunity Employer - Minorities/Women/Disabled/Veterans