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Rockefeller University Research Support Specialist in New York, New York

Job: IRC22485


Job Title Research Support Specialist

Laboratory / Department High Throughput Screening Resource Center

Department Description

The High-Throughput and Spectroscopy Resource Center (HTSRC) supports scientists in the screening and identification of compounds and genetic modulators of in vitro assays which utilize optical and other bio-analytical technologies. High-Throughput Screening (HTS) requires the miniaturization and automation of bioassays so that millions of variables can be tested. HTS is often an important step in the discovery of new medicines. The center has a collection of over 380,000 compounds, automated liquid transfer devices, compound databases, and supports a broad diversity of assay development techniques, typically found in early drug discovery programs. The Center is configured for processing 96, 384 and 1536-well microtiter plates through the use of automated changeable tip dispensers, non-contact dispensers, and syringe dispensers. For assay technologies, the HTSRC has the capability to support cellular and biochemical assays using absorbance, fluorescent kinetics, fluorescence anisotropy, time-resolved fluorescence, time-resolved fluorescence resonance energy transfer, AlphaScreen, bioluminescence, for example luciferase and green fluorescent protein, scintillation proximity and cellular fluorescence imaging. Assay targets can include ion channels, receptors, enzymes, protein interactions, signaling pathways and cellular processes.

The High-Throughput and Spectroscopy Resource Center also oversees the management of, training for, and scheduling for optical spectroscopic and calorimetric equipment for use in studies of the structure, function and interactions of biological and organic molecules. This includes an on-campus facility for nuclear magnetic resonance spectroscopy with priority access given to University researchers. The Center offers extensive training in basic and advanced NMR experiments and also coordinates access to high field spectrometers (700, 800, and 900 MHz) at the New York Structural Biology Center through the University's partnership in that Center. HTSRC staff provides general background training in applications of NMR spectroscopy to structural biology and chemistry and hands on training on the spectrometers. Trained users have 24/7 access to these instruments. Occasional users of the facilities may have spectra produced for them.

Detailed Description

Working as part of the High Throughput Screening Resource Center (HTSRC), the Research Support Specialist will be responsible for guidance in bioassay development, miniaturization and optimization of high throughput assays as well as running and oversight of the automated liquid handling instruments and robots. Will participate in the design of assay controls and screening experiments. The Research Support Specialist will conduct a high throughput screening project from assay miniaturization through confirmed hit list and SAR by inventory. This includes programming, maintenance, and quality control for the Perkin-Elmer Janus, TECAN EVO, Multi-drop Combi and the synergy NEO plate reader. Will Train users in the implementation of tool and drug discovery projects and be responsible for delivering formatted compound plates for single point concentrations and IC50 experiments. Additionally, will design and interpret experiments using biophysical techniques such as surface plasmon resonance, microscale thermophoresis, CD and ITC to measure the affinity of biomolecules for their receptors.

Job Requirements

Bachelor's degree in science required; Master's degree, Ph.D. and/or industrial experience with publications viewed upon favorably. A minimum of six years of post-graduate experience and extensive expertise with an HTS or spectroscopy related technology required. Expert technical knowledge of the development of HTS assays involving a wide variety of fluorescent and cell-based modalities necessary. Must possess a deep theoretical knowledge of chemical lead discovery and the associated technologies. Excellent communication skills , especially with clients a must. Must have mastery of analytical techniques such as NMR and HPLC-MS.

Additional Details